Hypertension and nephrology - 2016;20(01)

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Hypertension and nephrology

FEBRUARY 10, 2016

[Hypertensive urgencies and emergencies]

FARSANG Csaba

[Prompt recognition and evaluation of hypertensive urgencies and emergencies can substantially decrease definitive target organ damage and mortality. Most frequent cause of sudden increase in blood pressure is stopping antihypertensive treatment. In hypertensive crises quickly acting parenteral drugs are of choice, while in urgencies orally administered ones might be sufficient. Cerebral and coronary hypoperfusion should be avoided therefore blood pressure must be decreased gradually.]

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HUN

2016;20(01)

Hypertension and nephrology

FEBRUARY 10, 2016

[Incorporation of ortho- and meta-tyrosine into cellular proteins leads to erythropoietin-resistance]

MIKOLÁS Esztella Zsóka, KUN Szilárd, LACZY Boglárka, MOLNÁR Gergő Attila, SÉLLEY Eszter, KŐSZEGI Tamás, WITTMANN István

[Introduction: Erythropoietin (EPO) is a glycoprotein hormone, which is responsible for the proliferation and differentiation of erythroid cell lines. Since it is widely used as the treatment of renal anaemia, EPO-resistance is a common concern. Aims: We aimed to perform in vitro experiments to investigate a possible mechanism of EPO-hyporesponsiveness. Methods: We used a factor dependent erythroblast cell line (TF-1). Two independent observers calculated cell counts simultaneously on day 1; 2 and 3 in Bürker cell counting chambers. Colorimetric method was used to measure protein concentrations. Measurement of protein-bound para-, ortho- and meta-tyrosine was performed with reverse phase high performance liquid chromatography with fluorescence detection. We determined ERK and STAT5 activation using Western blot method. Results: In case of ortho- and meta-tyrosine pretreated cells time-dependent, EPOinduced proliferative activity was decreased compared to the 1.7 fold elevation of cell counts seen in para-tyrosine cultured cells. Protein concentration of ortho- and metatyrosine treated samples was significantly lower than control cells on the third day. Addition of para-tyrosine reclaimed EPO-sensitivity. Erythroblasts treated with orthoor meta-tyrosine contained lower concentrations of protein-bound para-tyrosine with higher ortho- and meta-tyrosine content. EPO dependent activation of ERK and STAT5 could be inhibited by ortho- or meta-tyrosine treatment. Conclusions: Elevated level of protein-bound ortho- and meta-tyrosine in erythroblasts can result in the pathological modification of intracellular signaling, leading to EPOhyporesponsiveness.]

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HUN

2016;20(01)

Hypertension and nephrology

FEBRUARY 10, 2016

[Prevalence and care of prediabetic patients in the Hungarian hypertensive population]

KÉKES Ede, KISS István

[Authors studies the incidence of hypertensive patients with prediabetes - according to the IFG criteria - based on the 2005 Hungarian Hypertension registry (38849 subject) and on the „Live under 140/90” sub-program („Prevent it”) (23670 subject) database. The presences of diabetes patients were excluded. IFG incidence (from 5,6 to 7,1 mmol/l) was found between 13-18% of the adult population over 40 years. In men, the incidence was significantly higher. In individuals with IFG the BMI, waist circumference values were significantly higher than the average normal values and exceeded the danger zone. For women significantly higher values were found. These patients had higher total cholesterol and blood pressure values. After three months of intensive supervision, counseling, care over every 40 years age group - without medication - significantly decrease the fasting blood glucose values. They presented the international experiences of prediabetes.]

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HUN

2016;20(01)

Hypertension and nephrology

FEBRUARY 10, 2016