Hypertension and nephrology

[The Classics of Hypertonology 5. – Professor Neil Poulter]

KÉKES Ede

FEBRUARY 10, 2016

Hypertension and nephrology - 2016;20(01)

COMMENTS

0 comments

Further articles in this publication

Hypertension and nephrology

[Commentary to “The EQUAL Study: a European Study in Chronic Kidney Disease Stage 4 Patients” Published by Jager et al.]

DEÁL György, PATÓ Éva

Hypertension and nephrology

[When Salt Intake is Restricted, Reduced Fructose Intake Does Not Reduce Blood Pressure Further, but Allopurinol Does]

VÁLYI Péter

Hypertension and nephrology

[Incorporation of ortho- and meta-tyrosine into cellular proteins leads to erythropoietin-resistance]

MIKOLÁS Esztella Zsóka, KUN Szilárd, LACZY Boglárka, MOLNÁR Gergő Attila, SÉLLEY Eszter, KŐSZEGI Tamás, WITTMANN István

[Introduction: Erythropoietin (EPO) is a glycoprotein hormone, which is responsible for the proliferation and differentiation of erythroid cell lines. Since it is widely used as the treatment of renal anaemia, EPO-resistance is a common concern. Aims: We aimed to perform in vitro experiments to investigate a possible mechanism of EPO-hyporesponsiveness. Methods: We used a factor dependent erythroblast cell line (TF-1). Two independent observers calculated cell counts simultaneously on day 1; 2 and 3 in Bürker cell counting chambers. Colorimetric method was used to measure protein concentrations. Measurement of protein-bound para-, ortho- and meta-tyrosine was performed with reverse phase high performance liquid chromatography with fluorescence detection. We determined ERK and STAT5 activation using Western blot method. Results: In case of ortho- and meta-tyrosine pretreated cells time-dependent, EPOinduced proliferative activity was decreased compared to the 1.7 fold elevation of cell counts seen in para-tyrosine cultured cells. Protein concentration of ortho- and metatyrosine treated samples was significantly lower than control cells on the third day. Addition of para-tyrosine reclaimed EPO-sensitivity. Erythroblasts treated with orthoor meta-tyrosine contained lower concentrations of protein-bound para-tyrosine with higher ortho- and meta-tyrosine content. EPO dependent activation of ERK and STAT5 could be inhibited by ortho- or meta-tyrosine treatment. Conclusions: Elevated level of protein-bound ortho- and meta-tyrosine in erythroblasts can result in the pathological modification of intracellular signaling, leading to EPOhyporesponsiveness.]

Hypertension and nephrology

[News of the Hungarian Society of Nephrology]

REUSZ György, BÖCSKEI Virág

Hypertension and nephrology

[Blood Pressure Target Values for Patients with Chronic Renal Disease]

SARAFIDIS A. Pantelis, RUILOPE M. Luis

All articles in the issue

Related contents

Ca&Bone

[István Holló, MD, professor 1926-2007]

SZŰCS János

Lege Artis Medicinae

[Ferenc Keresztúri, Professor of Medicine at the Moscow University ]

MOLNÁR László

Hungarian Radiology

[Loránd Fráter - A president without position; Professor Eberhard Willich is 85 years old; My memories on Hungarians]

LOMBAY Béla, SCHLÄFFER Erzsébet, EBERHARD Willich

Hypertension and nephrology

[Prominents in Hungarian nephrology Professor Gyula Petrányi (1912-2000). Part II]

SZALAY László

[A nation can only survive and keep its identity through its traditions. This is why the initiative to launch this series coming from professor János Radó is worthy of attention. Gyula Petrányi is an outstanding personality in 20th century internal medicine, to be more precise in nephrology and immunology, his activity being wide-ranging. The first part of the current summary of his work deals with a tribute to his personality, and his role in immunomodularity treatment in glomerulonephritis. The second part shall cover his role in spreading renal biopsy, screening and caring kidney patients, dialysis, in developing kidney patients’ care, furthermore in clinicopharmacology and renal transplantation.]

Lege Artis Medicinae

[“The Good Fame of our Guild Depends on Us” A Discussion with Rheumatology Professor Béla Gömör]

NEMESÁNSZKY Elemér