Lege Artis Medicinae

[The use of in situ hybridization in basic biomedical research and clinical medicine]

MIHÁLY András1

NOVEMBER 30, 1994

Lege Artis Medicinae - 1994;4(11)

[The essential purpose of in situ hybridization is the histochemical and/or autoradiographic detection of specific nucleic acid sequences in cells, tissues or chromosomes. The method is based on the use of artificially manufactured DNA or RNA fragments which are label led with isotope, enzymes, biotin or digoxigenin. The specific attachment of these labelled single-stranded nucleic acid probes to intra nuclear or intracytoplasmic nucleic acids can be used for the study of gene expression, for the establishment of viral infections, for the diagnosis of specific tumours and for the detection of hereditary diseases and chromosomal aberrations. The present review discusses the molecular basis of the method, the possibilities of labelling and detection and various other applications.]

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  1. SZOTE Anatómiai, Szövet és Fejlődéstani Intézet Szeged

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