Clinical Neuroscience

[Corticosteroid fractions in the human brain]

FAZEKAS I. Gyula1, FAZEKAS Attila2

APRIL 01, 1966

Clinical Neuroscience - 1966;19(04)

[In cases of 1) pneumonia, 2) sudden death from cardiac arrest, 3) sudden death from cardiac arrest in an alcoholic state, and 4) vehicle hit-and-run hemorrhage or spinal cord rupture, extracts were obtained from 5 brain areas of 10 people aged 21-72 years 2-9 hours after death and subjected to paper chromatography in a Bush 5 system. The extracts were run with the following corticosteroids as standard : 1. tetrahydrocortisol, 2. tetrahydrocortisone, 3. cortisol (hydrocortisone), 4. cortisone, 5. corticosterone, 6. 11-dehydrocorticosterone - Brain areas studied : 1. bridge + rabbit brain, 2. cerebellum, 3. cerebellar peduncles, 4. cerebral cortex, 5. cerebral white matter. 1. a total of 11 steroid variants were detected, 6 of which were identical to the standards, the other 5 unidentified steroids were designated X, (Rf. 0.03), X2 (Rf. 0.06), X3 (Rf. 0.19), X4 (Rf. 0.66), X5 (Rf. 0.70). 2. These steroids were detected in different amounts and qualities in different brain areas depending on the deaths. 3. The highest amounts of cortisol (hydrocortisone) and corticosterone were detected. Other fractions were present in varying numbers and qualities in different brain areas. 4. Most of the fractions and the highest amounts were found in the brain areas of those who died of pneumonia. This was followed by the brain areas of those who died suddenly due to heart failure. In third place were the brain areas of those who died of cardiac paralysis in an alcoholic state and in fourth place, i.e. the fewest fractions and the smallest amount of fractions were found in the brain areas of those who died of an accident in an alcoholic state. 5. In agreement with the results of our previous animal studies, it was found that the number and amount of corticosteroid fractions in all areas of the human brain were significantly lower after alcohol compared to those in those who died of other causes. 6. No seemingly regular order in the total steroids in the brain areas of those who died of pneumonia could be established. 7. In those who died suddenly from heart failure and in the hemorrhagic case, most steroids were found in the area of the bridge + rabbit brain, second was the steroid content of the cerebellum, third was the brainstem, fourth was the cerebral cortex and fifth was the white matter of the cerebrum. Only in the case of rabbit brain laceration was the steroid content of the rabbit brain + bridge and cortex (presumably due to the lesions) lowest compared to the other three brain areas. 8. Further studies are needed to determine whether age, sex, and the time between death and examination (more than 10 hours) influence the amount, number and size of corticosteroid fractions in each brain area. ]

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  1. Szegedi Orvostudományi Egyetem Igazságügyi Égészségügyi Intézete
  2. Szegedi Orvostudományi Egyetem Igazságügyi Egészségügyi Intézete

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