Clinical Neuroscience

[Further studies to detect human-brain corticosteroid fractions]

FAZEKAS I. Gyula1, FAZEKAS Attila1

DECEMBER 01, 1968

Clinical Neuroscience - 1968;21(12)

[1. Lobar pneumonia, sudden cardiac death from paralysis, sudden cardiac death from paralysis in alcoholic state, self-harm, lightning strike, apoplexy cerebri, carcinosis peritonei, isonicide poisoning, cyanide poisoning, barbiturate poisoning, CO poisoning, Wofatox poisoning, alcohol poisoning+freezing deaths 26-92 years of age from 5 brain areas of 18 people (11 males and 7 females) were extracted 1 1/2-10 h after death, paper chromatographed in Bush 5 system, followed by chromatography in 2 n NaOH and 0.1% tetrasolium blue 9: 1. Evaluation was based on the tetrasolium blue reaction, NaOH fluorescence, Rf. values, standards and native blue fluorescence. 2 Brain extracts were run with different amounts of tetrahydrocortisol, tetrahydrocortisol, cortisol, cortisone, corticosterone and 11-dehydrocorticosterone as standards. The brain areas tested were : 1. bridge+brain, 2. cerebellum, 3. cerebellar nuclei, 4. cerebral cortex, 5. cerebral white matter. 3. 10 corticosteroid fractions were identified in total, 6 fractions were all identical to the standards, the other 4 unidentified steroids were labelled X1, X2, X3, X4. 4. These steroids were detected in different qualities and quantities in different brain areas depending on the deaths. 5. cortisol (=hydrocortisone) was detected in almost all brain areas tested in all 18 cases. Cortisol was found in most brain areas of those who died of heart failure, self-harm, lightning, apoplexy cerebri and various poisonings, but could not be detected in brain areas of those who died of heart failure in an alcoholic state and of cyanide poisoning. Other fractions were present in varying numbers and quantities in different brain areas. 6. Most fractures and the largest number were found in the brain areas of those who died of carcinosis peritonei and pneumonia. Fewest fractures and least amount were found in brain areas of those who died of cardiac arrest in alcoholic state and various intoxications in alcoholic state, and alcohol poisoning + frostbite. 7. Consistent with our previous animal studies and our studies on human subjects, we observed in the present study that alcohol induced significantly fewer corticosteroid fractions and quantities in all areas of the human brain compared to those in the non-alcoholic state. 8. In the cases studied, the total steroid content of each brain area does not show a consistent quantitative pattern that is characteristic of each death. 9. No lawful correlations between age, sex, adrenal weight, body weight, body height and the quantity and quality of corticosteroid content in the brain were found. 10. No decrease in brain corticosteroid content within 10 hours after death was observed with increasing time.]

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  1. Szegedi Orvostudományi Egyetem Igazságügyi Orvostani Intézet

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