Clinical Neuroscience

[The Congress of the American Neuro-ophthalmological Society (NANOS)]

SOMLAI Judit

JUNE 20, 2002

Clinical Neuroscience - 2002;55(05-06)

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Clinical Neuroscience

[CONGRESS CALENDAR]

Clinical Neuroscience

[β-amyloid peptide-induced intracellular calcium level changes in Alzheimer fibroblasts]

PALOTÁS András, KÁLMÁN János, LASKAY Gábor, JUHÁSZ Anna, JANKA Zoltán, PENKE Botond

[Rationale - β-amyloid peptides, comprising the major neuropathological lesions of Alzheimer's disease, have been found to form depositions in various peripheral tissues, including the skin. Neurons in the disorder succumb to the altered ionic homeostasis and some other factors caused by this toxic peptide. In line with these findings, our study aimed to find differences in biochemical processes of cultured cutaneous fibroblasts derived from sporadic Alzheimer patients and from agematched control individuals that may mirror changes in the central nervous system. Methods - Intracellular ionic homeostasis of Alzheimer and control fibroblasts was measured in Fura-2AMloaded human fibroblasts by dual wavelength spectrofluorimetry. Results - Cells derived from Alzheimer patients exhibited lower intracellular free calcium levels as compared to the control cultures. Exposure of fibroblasts to β-amyloid resulted in increased calcium concentrations of the control cells, but not of Alzheimer ones. Conclusion - Our findings indicate that Alzheimer’s disease is a systemic disorder that, among others, affects the calcium homeostasis of fibroblasts. Even though it is unknown whether the diminished ionic response of Alzheimer fibroblasts is a disease or actual status marker, it could prove to be a useful model for the analysis of Alzheimer specific changes.]

Clinical Neuroscience

[Identification of gliomas by morphological and immunocytochemical analysis in cell cultures]

FAZEKAS Ilona, KEREKES Edit, HEGEDŰS Balázs, NYÁRY István

[Introduction - The morphology and immunocytochemical properties of 250 different monolayer cultures derived from various human brain tumor specimens were investigated on purpose to support and complement the neuropatholgical diagnosis. In this study analyses of 124 glioma cases are presented. Methods - The tumor samples were mechanically dissociated and seeded on glass coverslips. After the formation of the monolayer cultures were fixed and stained by May-Grünwald- Giemsa method for the morphological examination. Semi-quantitative immunocytochemical labeling included several different types of mono- and polyclonal primary antibodies using avidin-biotin visualization system. In nine cases of the glioblastomas the sufficient proliferation made possible to establish cell lines from the primary cultures. Results - The glial origin of the tumors was identified in 124 cases based upon the presence of glial fibrillary acidic protein. A negative correlation between the intensity of glial fibrillary acidic protein immunostaining and the grade of tumor malignancy was found. During long-term cultivation of the higher grade gliomas the incidence and intensity of glial fibrillary acidic protein labeled cells was decreasing. Both the vimentin and the neuron specific enolase labeling were in general stronger than the glial fibrillary acidic protein and almost all the cells were stained. The incidence of Ki-67 positive cells increased with the grade of malignancy. Concerning the tumor classification our immunocytochemical results correlated with the routine histopathological examination. Conclusions - On the basis of these results we conclude that monolayer cultures obtained from tumor specimens can support and complement the correct diagnosis of the various human brain tumors.]

Clinical Neuroscience

[Electrophysiological investigations in Parkinson’s diseae]

SZIRMAI Imre, GERTRÚD Tamás, TAKÁTS Annamária, PÁLVÖLGYI László, KAMONDI Anita

[Objective - Post-movement beta synchronization is an increase in EEG beta power after movement termination. Its characteristics in movement disorders are not well described. Tremor dominant Parkinson’s disease shows unique clinical, anatomical and biochemical features. In our study we examined the relation between the laterality of tremor and size of post-movement beta synchronisation in tremor dominant Parkinson’s disease. Methods - In a self-paced movement paradigm we measured movement duration and analyzed EEG power changes at movement-reactive beta frequencies. Results - Movement duration was significantly longer in Parkinson-patients than in controls (0.49±0.170 s, 0.35±0.087 s, p=0.013, Mann-Whitney test). There was no difference between the two hands in the control group (0.36±0.078s, 0.34±0.099 s, p=0.207, Wilcoxon-test), while Parkinson patients performed longer movement with their left hand (0.52±0.195 s, 0.46±0.148 s, p=0.049, Wilcoxon), unrelated to the side of tremor. In controls, post-movement beta synchronisation contralateral to the movement was not significantly different after right and left hand movement (108.1±68.21% and 92.1±23.43%, p=0.78 Wilcoxon). In Parkinson patients post-movement beta synchronisation was significantly smaller contralateral to the tremulous hand movement (36.9±47.79%, 104.7±91.42%, p=0.012, Wilcoxon-test). The post-movement beta synchronisation showed anterior shifting in Parkinson-patients. Conclusions - In tremor dominant Parkinson’s disease the asymmetric decrease of post-move beta synchronisation is related to the laterality of tremor rather than bradykinesia. Analysis of this phenomena might provide further insight to the pathophysiology of Parkinson’s disease.]

Clinical Neuroscience

[In remembrance of Kálmán Sántha, on his 45th death anniversary]

SZIRMAI Imre

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Clinical Neuroscience

Evaluation of anxiety, depression and marital relationships in patients with migraine

DEMIR Fıgen Ulku, BOZKURT Oya

Aim - The aim of this study was to evaluate the frequency and characteristics of attacks in patients with migraine, to determine the effects of anxiety or depressive symptoms, and to evaluate the marital relationships of patients with migraine. Method - Thirty patients who were admitted to the neurology outpatient clinic of our hospital between July 2018 and October 2018 and were diagnosed with migraine according to the 2013 International Headache Society (IHS) diagnostic criteria were included in this cross-sectional study. Age, sex, headache frequency and severity, depressive traits, marital satisfaction and anxiety status were examined. We used the Beck Depression Inventory (BDI), State-Trait Anxiety Inventory (STAI), Maudsley Marital Questionnaire (MMQ) and Visual Analogue Scale (VAS) for measuring relevant parameters. Results - The mean severity of migraine pain according to VAS scale was 6.93 ± 1.41 and the mean number of migraine attacks was 4.50 ± 4.24. The mean BDI score of the patients was 12.66 ± 8.98, the mean MMQ-M score was 19.80 ± 12.52, the mean MMQ-S score was 13.20 ± 9.53, the mean STAI-state score was 39.93 ± 10.87 and the mean STAI-trait score was 45.73 ± 8.96. No significant correlation was found between age, number of migraine attacks, migraine duration, migraine headache intensity, and BDI, STAI and MMQ scores (p>0.05). But there was a positive correlation between MMQ-S and scores obtained from the BDI and STAI-state scales (p<0.05). Conclusion - In this study more than half of the migraine patients had mild, moderate or severe depression. A positive correlation was found between sexual dissatisfaction and scale scores of depression and anxiety.

Clinical Neuroscience

[CONGRESS CALENDAR]

Hungarian Radiology

[23rd Congress of the Society of Hungarian Radiologists, 1st Hungarian-Austrian Congress of Radiology]

HARKÁNYI Zoltán

Lege Artis Medicinae

[The 8th Congress of the Hungarian Society of Thrombosis and Hemostasis]

BLASKÓ György

Journal of Nursing Theory and Practice

[The Importance of Teamwork and Patient Education in the Rehabilitation of Stroke Patients]

HORVÁTH Orsolya, STERLIK Krisztina

[The aim of the study: Stroke is an increasing problem in public health. Every year in Hungary tens of thousands of people survive stroke and continue their life bearing all the negative consequences of this disease. Well organized and early rehabilitation treatment, based on the patients’ clinical condition, improves not only their life expectancy, but also quality of life and helps to restore the self- sufficient living as well. The majority of the stroke patients live with numerous of cerebrovascular risk factors, highlighting the special importance of personalized education to prevent the recurrent stroke. Material and Methods: The aim of our investigation was to examine the efficiency of the neuro-rehabilitation teamwork and personalized patient-education among stroke patients took part inpatient rehabilitation of the Teaching Hospital and Rehabilitation Center of Sopron (2016-2017). We measured the change in ability of self- sufficient living with the FIM scale and the Bartel index, while we analyzed the presence of the modifiable cerebrovascular risk-factors with the assistance of a questionnaire was completed by the patient or the relatives (2016-2017). Results: During our research we also evaluated the knowledge of patients about their condition. According to our results the modifiable risk-factors occured cumulatively among our patients and despite the education that stroke patients received during the acute period in the hospital, there still occured a general lack of knowledge regarding their disease. Conclusions: Based on our results the complex rehabilitation therapy started at its earliest possible following the acute period was the most successful method of treatment. During the acute period, the dissemination of written educational materials and information sheets help the recovery phase until the start of the complex rehabilitation therapy. ]